WashU gRNA Designer for CRISPR/Cas9 Knockout
Option 1: Search for predesigned gRNA to target human or mouse genes:
|Search by gene target|
Option 2: gRNA design for user-provided genomic target sequence:
All the gRNAs in WU-CRISPR are designed by a bioinformatics tool, sgDesigner. Custom genomic target sequences must be between 26 and 30,000 bases long. Please submit only one sequence at a time, consisting of A, T or U, C and G. Please note that all spaces, line breaks, and numbers within the submission will be removed, and multiple lines will be joined together to create a single sequence.
The stand-alone gRNA design tool can be downloaded here. This program runs under a Red Hat-compatible Linux system.